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Mixture of histones H1 H2A H2B H3 and H4 isolated from calf thymus.Histones are a group of DNA-binding proteins that are characterized by relatively high levels of lysine and arginine. five different fractions of histones have been isolated and characterized. These are named as H1 H2A H2B H3 and H4. The H1 fraction is lysine-rich while the H2A and H2B are slightly lysine-rich The H3 and H4 fractions are arginine-rich. The molecular weights of histones are approximately 11 to 21 kDa depending on the fraction.
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The Ultra II DNA Library Prep Kit for Illumina meets the challenge of constructing high quality libraries from ever-decreasing input quantities. The reagents for each step in the library preparation workflow have been reformulated to enable high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA. This new generation of NEBNext reagents uses a fast, streamlined, automatable workflow and enables use of fewer PCR cycles while also improving GC coverage. The kit is also compatible with PCR-free workflows and is effective with challenging samples such as FFPE DNA.
Get more of what you need, with the highest library yields
Use to generate high quality libraries from a broad range of input amounts, from 500 pg to 1 µg
Prepare libraries from ALL your samples, including GC-rich DNA and FFPE DNA samples
Improve yields and quality for target enrichment applications
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The pCMV-ProLabel C Vector is a mammalian cloning vector for use in expressing a protein of interest fused to the ProLabel (ProLabel) tag at the C-terminus. The ProLabel tag is a version of ED used for EFC assays (in combination with EA) where ED and EA are contained in separate cellular compartments. Provided as a vial of 10 μg vector in 20 μL liquid buffer. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/reagent/vector/pcmv-c-vector-93-0012.
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